Component	Final concentration	Stock	Amount
Sodium Phosphate Monobasic	9.4 mM	1 M	94 μL
Sodium Phosphate Dibasic	40.6 mM	500 mM	812 μL
MgCl2	10 mM	1 M	100 μL
Ampicillin	2 mg/mL	100 mg/mL	200 μL
DTT	10 mM	1 M	100 μL
dATP	0.5 mL	100 mM	50 μL
Creatine Phosphate	100 mM	500 mM	2000 μL
Uracil	8 mM	50 mM	1600 μL
Bovine serum albumin	0.1 mg/mL	10 mg/mL	100 μL
Creatine Kinase	0.005 mg/mL	1 mg/mL	50 μL
Myokinase	0.010 U/μL	5 U/μL	20 μL
Pyrophosphatase	0.004 U/μL	2 U/μL	20 μL
RK	0.005 U/μL	0.5 U/μL	100 μL
PRPPS	0.0003 U/μL	0.01 U/μL	300 μL
UPRT	0.005 U/μL	0.4 U/μL	125 μL
Add 4.23 mL water to 9.9 mL final volume