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. Author manuscript; available in PMC: 2018 Apr 2.
Published in final edited form as: Methods Enzymol. 2014;549:133–162. doi: 10.1016/B978-0-12-801122-5.00007-6
Component Final concentration Stock Amount
Tris-HCl pH 8.0 50 mM 1 M 500 μL
MgCl2 10 mM 1 M 100 μL
Ampicillin 2 mg/mL 100 mg/mL 200 μL
dATP 4 mM 100 mM 400 μL
UTP 2 mM 50 mM 400 μL
CTPS 0.10 mg/mL 9.6 mL 17.8 mg/mL 56.2 μL
Add 7.94 mL water to final volume