Chemo-enzymatic synthesis of selectively 13C/15N-labeled RNA for NMR structural and dynamics studies

. Author manuscript; available in PMC: 2018 Apr 2.

Published in final edited form as: Methods Enzymol. 2014;549:133–162. doi: 10.1016/B978-0-12-801122-5.00007-6


Component	Final concentration	Stock	Amount

UTP/CTP	1 mM	50 mM	13 μL

D₂O	10% (v/v)	100% (v/v)	65 μL

DSS	0.1 mM	1 mM	65 μL

Add ddH₂O to 650 μL and transfer to a regula r-volume NM R tube