Component	Final concentration	Stock	Amount
Transcription Buffer	1X	10X	1000 μL
MgCl2	Varies	1 M	Varies
PEG	80 mg/mL	400 mg/mL	2000 μL
DNA Template	0.3 μM	10 μM	300 μL
DTT	0.01 M	1M	100 μL
TIPP	2 Units/mL	2000 Units/mL	10 μL
ATP	Varies	100 mM	Varies
GTP	Varies	100 mM	Varies
Specifically Labeled UTP	Varies	100 mM	Varies
Specifically Labeled CTP	Varies	100 mM	Varies
T7 RNA Polymerase	0.10 mg/mL	10 mg/mL	100 μL
Add water to 10 mL