Fig. 4.

Blockage of Wnt/β-catenin/MYC/Sox2 axis abrogates the RU to RR conversion. a Pharmacological inhibition of MYC or β-catenin using 5 μM 10074-G5 or 50 μM quercetin for 24 h significantly decreased the SRR2 luciferase activities that were upregulated by H₂O₂ re-challenge. The Western blots below showed the knockdown efficiency of MYC and β-catenin after inhibitor treatment for 24 h. b RU cells with Sox2 knockdown by siRNA showed significantly decreased SRR2 luciferase activity in comparison to cells transfected with scrambled siRNA in the H₂O₂ re-challenge experiment. The Western blots below suggested the Sox2 knockdown efficiency at 48 h post siRNA transfection. Imag J software was used to analyze the densitometry. All proteins’ densitometry values were normalized to γ-tubulin bands, and the densitometry value of protein of interest in RU cells was normalized as 1.0