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. 2018 Apr 2;13:8. doi: 10.1186/s12263-018-0596-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Evaluation of the microRNA-16-1/survivin mRNA, p53/survivin, and caspase-3/survivin protein ratios in DHA-treated cells. 5 × 10⁵ HCT-116 cells/well were treated with different DHA concentrations for 48 h and microRNA-16-1, p53, survivin, and caspase-3 expression levels were measured. Thereafter, microRNA-16-1/survivin mRNA, p53/survivin, and caspase-3/survivin protein ratios were calculated. The results were presented as mean ± SD of two independent experiments with triplicate assays. *P < 0.05 and **P < 0.01 vs. untreated control cells