Fig. 2.

SGK1 inhibition impairs PCa cells migration and invasion in vitro. a Wound healing assays of PC3 cells treated with DMSO or 40 μM GSK650394. Phase-contrast images were acquired at 0, 24 and 48 h after scratching and representative images of three independent experiments are shown. The wound healing area was analyzed by using ImageJ software and the corresponding data, relative to 0 h, expressed in the graph (b). c Cell migration assays were performed on SGK1-depleted PC3 and LNCaP cells (shSGK1) and on control cells (LV2-Ctrl). Representative fields of migration cells on the membrane are shown (magnifications, × 200). Average migration cell number per field is quantified. d Matrigel cell invasion assays were performed as in c. Representative images show the cells that invaded through the matrigel. Representative histograph of invaded tumor cells is displayed and number of invaded tumor cells quantified. * indicates significant difference from the controls (* P < 0.05, ANOVA)