Fig. 4.

Molecular characterization reveals higher cytokine expression potential of CD96^low Th9 cells. (A) Intracellular production of IL-9 and IL-4 in CD96^high and CD96^low in vitro differentiated alloreactive C57BL/6 Th9 cells (n = 13). P values were calculated with a one-tailed Wilcoxon matched-pairs signed rank test. (B) mRNA expression levels of Il9 and transcription factors related to Th9 differentiation and (C) mRNA expression levels of additional genes of interest in CD96^high and CD96^low cells sorted from in vitro differentiated alloreactive C57BL/6 Th9 cultures analyzed by real-time qRT-PCR. Results were compared with expression in total CD44⁺ Th9 cells and, if indicated, with total CD44⁺ unpolarized cells (w/o, n = 2). Statistical analyses of Th9 datasets (n = 5) were performed by applying Friedman’s test and Dunn’s multiple comparisons test. qRT-PCR, quantitative reverse transcription–PCR. n.s., not significant (P > 0.05).