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PPK2 mechanism and interaction with polyP. (A) Proposed mechanism of FtPPK2 and the modified nucleotide-binding mode for MrPPK2 that permits phosphorylation of the α-phosphate (highlighted in red). Blue curly arrows indicate the forward reaction (ATP formation), red curly arrows the reverse reaction, and black curly arrows are common to both. (B) Structure of FtPPK2 Asp117Asn variant cocrystallized with polyP. Labels indicate the polyP lengths: P6, P11, and P24.
