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Cryo-EM structure of the AlfA filament. (A) The two-stranded AlfA filament with a pitch of 394 Å. The pointed end is at top and barbed end at bottom throughout all of the figures. (B) The atomic model fit into a segment of cryo-EM density. (C) A single AlfA protomer from the reconstruction colored by subdomain. (D) Protomers of different actin filaments, with bound nucleotides in yellow, share a conserved fold, but AlfA is missing subdomain IIb.
