Fig. 3.

MYB3R4 is not part of the TSO1-MYB3R1 module. (A) tso1-1 plant. (B) tso1-1; myb3r4^CR1 plant exhibiting the same phenotype as tso1-1 single mutant. The specific mutations in MYB3R4^CR1 are shown in E. (C) tso1-1; myb3r1-5 plant with a mild phenotype (reduced fertility). (D) tso1-1; myb3r1-5; myb3r4^CR2 plant showing a similar fertility defect (small siliques) as the plant in C. The specific biallelic sequence changes in MYB3R4^CR2 are shown in E. (E) MYB3R4 gene structure and CRISPR-edited mutations. R1R2R3 coding regions are underlined. The red sequence in the second exon of MYB3R4 was targeted by the gRNA. (Scale bars in A–D, 1 cm.)