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. 2018 Mar 12;115(13):3374–3379. doi: 10.1073/pnas.1717116115

Fig. 5.

Fig. 5.

Papi specifically interacts with Piwi in vivo. (A) Co-IP of Flag-tagged wild-type or mutant Papi with Myc-Piwi in S2 cells. (B) MS analysis for Papi-eTud–associated proteins in the fly ovary. (C) Fertility test (number of eggs laid) in w1118, papi−/− and papi mutant rescue flies. (D) Transposon expression levels of ovaries from w1118, papi−/−, and papi mutant rescue flies were quantified by RT-PCR and normalized to rp49. (E) Dot blot assay showing the selectivity of anti–Piwi-R10me2s antibody against various Piwi peptides. (F) Papi-eTud is specific associated with Piwi in Drosophila ovaries, and Piwi-R10me2s exists in fly ovaries. (G) Model of the role of Papi in the piRNA 3′-end trimming downstream of Zuc via direct interaction with the Piwi N terminus by the Papi-eTud domain.