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Overview of the milling procedure. (A) Flowchart depicting the procedure for image-guided tissue extraction. (B–D) The temperature measured during the entire procedure with sensors in the stomach, in the rectum, and embedded under the skin is depicted below for freezing and embedding (B), at the time when the mouse is placed at room temperature covered with dry ice (C), and during milling (D). (E) An example of NMR spectra from samples obtained with the IGMM.
