Table 1.

Target purification of the thiolase/HMGCS complex

Purification step	Protein, mg	Total activity, U	Specific activity, U/mg	Purification, -fold
Soluble fraction	4,000	31	0.008	1
DEAE Sepharose	2,300	99	0.043	5.4
Q Sepharose	1,100	130	0.12	15
Hydroxyapatite	310	59	0.19	24
Source 15 Phe	4.3	43	10	1,300

The activity was determined using HMGCR-coupled assay. The assays contained 2 mM acetyl-CoA, 2 µM HMGCR, and 450 µM NADPH in 100 mM Hepes-NaOH pH 7.5.