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. 2018 Mar 12;115(13):E2960–E2969. doi: 10.1073/pnas.1720696115

Fig. 2.

Fig. 2.

Chronic stress disturbed the immune system. (A) Flow cytometric analysis of B cells (CD45+, CD19+), NEUs (CD45+, Ly6G+), and ly6chi macrophages in colonic lamina propria (n = 4–5). (B) Representative F4/80 staining images and mean positive cells counted in five high-power fields (HFPs; n = 4–5). (Scale bar: 20 μm.) (C) Thymus coefficient, MLN coefficient, and spleen coefficient (n = 9–10). Thymus/MLN/spleen coefficient is calculated as thymus/MLN/spleen weight/body weight × 100. (D) WBC count in peripheral blood (n = 4–5). (E) Flow cytometric analysis of B (CD45+, CD19+), CD4+ T (CD45+, CD4+), CD8+ T (CD45+, CD8+), and NEU (CD45+, Ly6G+) cells in MLN (n = 4–5). (F) RT-PCR and ELISA results of IL-6 in colon (n = 5). (G) Western blot results of p-STAT3 in colon (n = 5; *P < 0.05, **P < 0.01, and ***P < 0.001).