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. 2018 Mar 12;115(13):E2988–E2996. doi: 10.1073/pnas.1721916115

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Fig. 4. — Effects of PT on in vitro transcription. (A) Schematic of the approach used to prepare DNA templates. In vitro transcription (B) and assessment (C) of the impact of PT on transcriptional efficiency using real-time PCR and the comparative Ct method. The Ct and fold change values represent the average values of Ct ± SD and gene expression ± SD from three independent experiments, respectively. The text in bold in B indicates genes with an expression ratio greater than twofold, with an FDR ≤ 0.001.