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. 2018 Apr 2;15:60. doi: 10.1186/s12985-018-0970-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Expression and characterization of DENV1–4 VLPs. Purified virus and VLPs were subjected to SDS-PAGE and analyzed with (a) CBB and (b) WB using anti-E 1 M7 and anti prM 2G3 mAbs. E-dimers (E^D), E-monomers (E^M), prM and capsid (c) proteins are indicated. The prM/E ratio is indicated below panel B and is determined using ImageJ software