Fig. 5.

DENV infected patient sera is efficiently depleted by DENV VLPs. Purified DENV particles or DENV VLPs were coated onto magnetic beads and incubated with convalescent patient sera. BSA was used for the depletion handling control. a DENV specific IgG levels for all depletion samples were determined by ELISA. b DENV specific neutralizing antibody titers were analyzed by a Vero-cell flow cytometry based neutralization assay. Neutralizing titers are expressed as Neut₅₀ values indicating the serum dilution where 50% of the virus is neutralized