Fig. 4.

3′ untranslated regions allow discrimination between closely related coding sequences that are differentially expressed. a Gbrowse image showing mapping coverage of the locus encompassing the adenylate cyclases AC320 and AC330. 3’ UTRs are underlined in blue. Early: early procyclic forms; late: late procyclic forms. b Scatter plot of transcripts showing ≥2-fold differences between early and late procyclic forms, based on mapping to their 3’ UTRs. A comprehensive list is provided in Additional file 5. The scale is enlarged to show weakly expressed genes, resulting in the exclusion of GPEET. HK1: hexokinase 1; HK2: hexokinase 2; AC330, adenylate cyclase (Tb927.5.330); CF: calflagin (Tb927.8.5440); PTT, putative pteridine transporter; REC: RNA editing complex; Hypo: Hypothetical protein (Tb927.10.10000); EP3–2: EP procyclin; AATP: amino acid transporter (Tb927.11.6680); VP2: vacuolar proton pyrophosphatase 2 (Tb927.8.7980); FBPase: fructose-1,6-bisphosphatase (FBPase) (Tb927.9.8720); PF: prostaglandin f synthase (Tb927.11.4700); NP: nitrogen permease regulator 2 (Tb927.7.3010). RPM: reads per million