Table 2.
Differential abundance of proteins (SILAC) and transcripts (RNA-Seq) in early and late procyclic forms
| Up in early procyclic forms | |||||
| Gene ID | Description | SILAC rep1 | SILAC rep2 | RNA-seq Rep1 | RNA-seq Rep2 |
| Tb927.8.5460 | Calflagin (flagellar calcium-binding protein 44) | 8.4 | 3.59 | 5.75 | 5.01 |
| Tb927.8.2210 | Pteridine reductase (PTR1) | 3.07 | ND | 1.96 | 2.12 |
| Tb927.10.3660 | Aspartate aminotransferase | 3.26 | 3.18 | 2.35 | 2.95 |
| Tb927.10.2010 | Hexokinase (HK1) | 3.4 | 2.93 | 4.48 | 3.74 |
| Tb927.11.4700 | Prostaglandin f synthase | 2.37 | 2.4 | 4.94 | 3.81 |
| Tb927.9.7000 | Methyltransferase domain containing protein, putative | 2.61 | 2.93 | 2.54 | 2.24 |
| Tb927.9.6090 | PTP1-interacting protein, 39 kDa | 2.74 | 2.28 | 2.40 | 2.10 |
| Tb927.1.2100 | Calpain-like protein 1.1 (CALP1.1) | 1.09 | 2.03 | 2.70 | 2.60 |
| Tb927.7.7500 | Thymine-7-hydroxylase, putative (TLP7) | 4.08 | 1.97 | 3.52 | 2.44 |
| Up in late procyclic forms | |||||
| Gene ID | Description | SILAC rep1 | SILAC rep2 | RNA-seq Rep1 | RNA-seq Rep2 |
| Tb927.1.3950 | ALAT | 2.44 | 1.68 | 3.45 | 0.85 |
| Tb927.9.8720 | FBPase | 2.61 | 3.29 | 2.78 | 2.94 |
| Tb927.7.190 | Metallo-peptidase | 2.77 | 2.58 | 1.40 | 1.67 |
| Tb927.11.2500 | Carboxypeptidase | 2.46 | 2.72 | 2.13 | 1.79 |
| Tb927.11.16550 | Zinc finger protein, C3H1 type-like (ZC3H46) | 8.78 | 33.24 | 1.0 | 3.03 |
| Tb927.9.4200 | Fatty acyl CoA synthetase 2 (ACS2) | 2.19 | 2.07 | 1.43 | 1.70 |
| Tb927.10.14140 | Pyruvate kinase 1 (PYK1) | 2.38 | 2.00 | 1.41 | 1.40 |
Numbers denote fold changes. Two biological replicates were performed in each case. The data for SILAC are derived from Imhof et al. [6]. ND not detected