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. 2018 Feb;13(2):257–264. doi: 10.4103/1673-5374.226396

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Copyright: © Neural Regeneration Research

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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Pathological changes and apoptotic cells in the injured brain.

(A) Photographs of brain sections stained with hematoxylin and eosin 3 days after reperfusion. Suspended moxibustion for 35 minutes alleviated the pathological changes. The arrows indicate cell loss. Scale bars: 100 μm. (B) Photographs of apoptotic cells (arrows) detected by the TUNEL assay 3 days after reperfusion in rats. The 35-minute moxibustion treatment decreased the total area of TUNEL-positive cells compared with the I/R and 15-minute moxibustion groups. Scale bars: 100 μm. (C) Quantitative analysis of the percentage of TUNEL-positive cells, expressed as the mean ± SEM (n = 3) and analyzed by one-way analysis of variance followed by Dunnett's test. #P < 0.05, vs. sham group; *P < 0.05, vs. I/R group; ΔP < 0.05, vs. M15 group. Sham: Sham surgery group; I/R: I/R group; M15: 15-minute moxibustion group; M35: 35-minute moxibustion group. I/R: ischemia/reperfusion; TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling.