Figure 1.

SB216763 promoted gap closure in RSC96 cells.

(A–C) Control cells at 0, 24 and 48 hours. (D–F) SB216763-treated cells at 0, 24 and 48 hours. Scale bars: 200 μm. (G) Relative area in the gap of wound at each time. At 24 hours after seeding, confluent RSC96 cells were treated with SB216763 to conduct the scratch assay. Relative area in the gap of wound was determined at 24 and 48 hours after scratching. As a result, SB216763 significantly promoted gap closure as compared with the control (**P < 0.01, vs. control group; mean ± SD, n = 3, Student's t-test). Three independent experiments were performed.