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. 2018 Mar 26;11:92. doi: 10.3389/fnmol.2018.00092

Figure 3.

Figure 3

The effects of BNIP3 on GSC malignant behavior in U87-GSCs and U251-GSCs treated with EMAP-II and TMZ. (A) Effects of EMAP-II and TMZ on BNIP3 expression in U87-GSCs and U251-GSCs, as demonstrated by western blotting. The combination of EMAP-II and TMZ significantly increased the level of BNIP3 expression. Data are presented as the mean ± SD of n = 3. *P < 0.05, **P < 0.01 vs. control group, #P < 0.05 vs. TMZ group, &P < 0.05 vs. EMAP-II group. (B) The effects of BNIP3 on U87-GSC and U251-GSC proliferation were assessed by CCK-8. BNIP3 overexpression inhibited GSC proliferation. Data are presented as the mean ± SD of n = 3. *P < 0.05 vs. BNIP3(+)-NC group, #P < 0.05 vs. BNIP3(–)-NC group. (C) The effects of BNIP3 on U87-GSC and U251-GSC migration and invasion were assessed by transwell assays. BNIP3 overexpression inhibited GSC migration and invasion. Data are presented as the mean ± SD of n = 3. *P < 0.05, vs. BNIP3(+)-NC group, #P < 0.05 vs. BNIP3(–)-NC group.