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. 2018 Mar 26;11:92. doi: 10.3389/fnmol.2018.00092

Figure 5.

Figure 5

The effects of miR-24-3p on mitophagy-related protein expression levels and U87-GSC and U251-GSC malignant behavior. (A) Effects of EMAP-II and TMZ on MiR-24-3p expression, as demonstrated by qRT-PCR, in U87-GSCs and U251-GSCs. MiR-24-3p expression levels were obviously decreased in the EMAP-II and TMZ combination group. Data are presented as the mean ± SD of n = 3. *P < 0.05, **P < 0.01 vs. control group, #P < 0.05 vs. TMZ group, &P < 0.05 vs. EMAP-II group. (B,C) Western blot analysis of the effects of miR-24-3p overexpression and silencing on the LC3-II/I ratio and BNIP3, P62, TOMM 20, and CYPD expression levels. MiR-24-3p silencing up-regulated LC3-II/I ratio and BNIP3 expression and reduce P62, TOMM 20, and CYPD expression. Data are presented as the mean ± SD of n = 3. *P < 0.05 vs. agomir-24-3p-NC group, #P < 0.05 vs. antagomir-24-3p-NC group. (D,E) The effects of miR-24-3p on U87-GSC and U251-GSC proliferation, migration, and invasion were assessed by CCK-8 or transwell assays. MiR-24-3p silencing inhibited GSC proliferation, migration, and invasion. Data are presented as the mean ± SD of n = 3. *P < 0.05 vs. agomir-24-3p-NC group, #P < 0.05, ##P < 0.01 vs. antagomir-24-3p-NCgroup.