Fig. 2.

Fatty acids production in E. coli strain harboring TE10. a Fatty acids profile of E. coli MG1655 strains without (upper) and with (lower) pJMYEEI82564 plasmid carrying thioesterase TE10 from Anaerococcus tetradius. b FFAs concentration and distribution in E. coli strain MG1655 harboring TE10. Upper, final free fatty acid titers at 72 h; lower, % mass percentage of produced fatty acids. Cultures were performed in 40 mL M9 + 1.5% (wt/v) dextrose in 250-mL shake flasks at 250 rpm 30 °C with an initial pH of 7.0, IPTG of 1 mM. Titers are the average of at least three biological replicates at 72 h with error bars indicating one standard deviation. TE10, thioesterase from Anaerococcus tetradius