Figure 8.

MiR-194 regulated the development and differentiation of inner ear sensory patches by modulating the expression of Fgf4. The percentage of Sox2 (left panel) and acetylated-tubulin (right panel) were measured by in situ hybridization at 48 and 72 hpf. The white circle area indicated zebrafish inner ear. Red staining indicated Sox2 expression (left panel) or acetylated-tubulin expression (right panel); blue indicated cell nucleus. MO – microinjection with morpholino oligonucleotide; NC – negative control; MO+FGF4 – microinjection with morpholino oligonucleotide and Fgf4; hpf – hour post fertilization.