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. 2017 Dec 22;11(2):99–115. doi: 10.1007/s12195-017-0517-4

Figure 1.

Figure 1

Schematic representation of the experimental procedure. rBMSCs were aggregated via the hanging drop technique as previously reported and harvested after 24 h. Subsequently, spheroids were plated onto HA-coated 96 well plates and cultured for 0, and 7 days. The individual spheroids were subjected to five different culture medium conditions, namely Dulbecco’s modified eagle medium (DMEM) with (a) 5 ng/mL TGF-β3, (b) 100 ng/mL IGF, (c) 40 μg/mL chondroitin sulfate (d) 40 μg/mL aggrecan, or (e) control medium, and (f) TGF ⇒ IGF medium.