Table I. O-glycans released from tryptic peptides of S. sputigena C9LY14 flagellin using reductive β-elimination. Released O-glycans were detected using PGC nanoLC-ESI MSMS. RT, retention time in minutes; all mass values are in Dalton; calc. m/z, theoretical m/z value. Structures of O-glycans in bold letters were determined based on MSMS spectra in negative ion mode. For all others, no product ion spectrum was generated, assignment based on MS1 level only.

RT	m/z	z	calc. m/z	Δm/z	Composition	Modification	Relative abundance [%]
24.7	457.16	1	457.19	-0.03	Rha3		7.6
28.3	749.34	1	749.31	0.03	Rha5		59.0
39.9	763.34	1	763.32	0.02	Rha5	+14 Da	0.9
38.6	791.36	1	791.32	0.04	Rha5	+42 Da	0.5
24.5	660.30	1	660.27	0.03	Rha3 GlcNAc		3.8
26.1	660.30	1	660.27	0.03	Rha3 GlcNAc		8.4
29.5	660.30	1	660.27	0.03	Rha3 GlcNAc		19.3
37.9	702.31	1	702.28	0.03	Rha3 GlcNAc	+42 Da	0.5