Table II. Summary of all 22 unique glycopeptide species identified from S. sputigena flagellin C9LY14. Identified glycosylation sites are indicated in bold, red letters. RT, retention time in minutes; all mass values are in Dalton. * Indicates glycopeptides where the site of glycosylation could not be unambiguously identified.

RT	m/z	z	calc. m/z	Δm/z	Peptide	Glycan	Modification
23.2	785.42	2	785.36	-0.06	191VNQGKTETTSF201	Rha2	+14 Da +52.1 Da
23.4	842.48	2	842.39	-0.09	179NIHSQDTITVSY190	Rha2	+14 Da
25.8	956.02	2	956.04	0.02	179NIHSQDTITVSY190*	Rha2	+14 Da +227.3 Da
25.1	1164.09	2	1164.08	-0.01	326AQALGLQGSDGSTLNISTR344	Rha3
25.4	1171.08	2	1171.07	-0.01	326AQALGLQGSDGSTLNISTR344	Rha3	+14 Da
25.3	1310.12	2	1310.13	0.01	326AQALGLQGSDGSTLNISTR344*	Rha5
16.1	581.79	2	581.77	-0.02	196TETTSFK202	RhaGlcNAc
17.0	561.28	3	561.23	-0.05	191VNQGKTETTSF201	GlcNAc	+267.1 Da
17.4	780.84	2	780.87	0.03	191VNQGKTETTSF201	RhaGlcNAc
17.7	787.84	2	787.87	0.03	191VNQGKTETTSF201*	RhaGlcNAc	+14 Da
18.8	801.91	2	801.92	0.01	191VNQGKTETTSF201	RhaGlcNAc	+42 Da
18.9	947.92	2	947.93	0.01	191VNQGKTETTSF201	Rha3GlcNAc	+42 Da
19.2	780.88	2	780.87	-0.01	142NQKVTATTTTF152*	RhaGlcNAc
20.1	801.86	2	801.94	0.08	142NQKVTATTTTF152	RhaGlcNAc	+42 Da
20.5	947.92	2	947.95	0.03	142NQKVTATTTTF152	Rha3GlcNAc	+42 Da
20.9	742.43	3	742.40	-0.03	136LVDGTHNQKVTATTTTF152*	RhaGlcNAc	+42 Da
24.3	842.94	2	842.93	-0.01	256AAGTGVGKSVGGITF270	RhaGlcNAc	+14 Da
24.5	982.05	2	981.99	-0.06	256AAGTGVGKSVGGITF270	Rha3GlcNAc
24.7	835.97	2	835.93	-0.04	256AAGTGVGKSVGGITF270	RhaGlcNAc
24.9	856.89	2	856.98	0.09	256AAGTGVGKSVGGITF270	RhaGlcNAc	+42 Da
24.9	1003.08	2	1002.99	-0.09	256AAGTGVGKSVGGITF270	Rha3GlcNAc	+42 Da
25.3	724.35	2	724.34	-0.01	245TASGESAISLY255*	RhaGlcNAc