Fig. 3.

Reproducibility of ion-current-based quantitation. (A) Evaluation of the analytical reproducibility for the ion-current-based quantitation method. The log2 intensity of proteins from two replicate runs of the same sample was compared and the reproducibility of ion-current-based quantitative data was evaluated by linear regression. (B) Box plot (10–90 percentile) of coefficients of variation of protein intensities in each treatment group and technical replicate. Median CV in technical replicates (n = 4) was 3% and CV of biological replicates (n = 3) was measured to be <20%.