Fig. 1.

Workflow for the isolation and proteomic and transcriptomic analyses of the centipede. The centipede Scolopendra subspinipes mutilans was used for proteomic and transcriptomic analyses. Venoms and torso tissues were processed individually and subjected to SDS-PAGE followed by in-gel digestion. Then, samples were analyzed in a separate ESI-MS/MS assay. For the transcriptomic analysis, venom glands, not venoms, and torso tissues were used for high-throughput sequencing. The functional analysis was combined with proteomic and transcriptomic data.