Figure 2.

RABEP2 regulates VEGFR2 signaling. A, HUVEC were serum-starved for 12 h (0 min) and then stimulated with 50 ng/ml of VEGF-A₁₆₅ for 5, 15, or 30 minutes (min). Protein lysates were collected and analyzed by Western blot using antibodies against the tyrosine 1175 phosphorylation site of VEGFR2 (pVEGFR2 Y1175), total VEGFR2, the serine 473 phosphorylation site of AKT (pAKT S473), total AKT, phosphorylated ERK1 and -2 (pERK1/2), total ERK1 and 2 (ERK1/2), RABEP2, and GAPDH as a loading control. B, quantification of pVEGFR2 (Y1175) normalized to total VEGFR2 levels. C, quantification of pAkt (S473) normalized to total Akt. D, quantification of pERK1/2 normalized to total ERK1/2. B–D, quantification was based on 4 independent experiments (mean ± S.D., *, p < 0.05).