Skip to main content
. Author manuscript; available in PMC: 2018 Apr 2.
Published in final edited form as: Plant Cell Rep. 2018 Feb 1;37(3):501–513. doi: 10.1007/s00299-017-2245-6

Fig. 1.

Fig. 1

Plants lacking DDM1 display precipitous telomere shortening in the sixth generation. a TRF analysis of bulk telomere length of wild-type (WT), G2 and G6 ddm1-2 mutants is shown. DNA from an individual plant is analyzed in each lane. b TRF analysis of bulk telomere length in different generations of ddm1-2 mutants is shown. Some variation of telomere length is observed among individuals. Arrows indicate more homogeneous TRF bands within a given individual and may represent telomere recombination events. c Telomere length was assessed on individual chromosome arms by primer extension telomere repeat amplification (PETRA). 1L and 5R indicate PETRA analysis using subtelomeric primer for the left arm of chromosome 1 or right arm of chromosome 5, respectively. PETRA results for the two chromosome arms (1L and 5R) of an individual plant are shown in the top and bottom panel. d TRF analysis of G1 tert and G5 and G6 ddm1 mutants. Results for offspring of a tert heterozygous plant are analyzed (lanes 1–3). The bracket indicates telomeres below the 2 kb threshold