Figure 2.

Findings from molecular genetic analysis of tumor tissue. Chromosomal microarray performed on the proband's astrocytoma tissue. A complex pattern of gains and losses is observed. Key features include deletion of CDKN2A and loss of heterozygosity for 17p (A). Sanger sequencing. Top trace shows wild-type control, central trace shows the maternal gastric tumor with heterozygous pattern attributed to mixture of normal and tumor cells. The proband's astrocytoma with normal TP53 allele loss is shown in the lower trace, marked by an arrow (B). Pedigree from the proband's immediate family (C). Solid symbols (squares, males; circles, females) indicate clinically affected individuals; slashed symbols indicate deceased individuals. Green, Li–Fraumeni syndrome; red, metastatic choriocarcinoma.