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. Author manuscript; available in PMC: 2018 Apr 2.
Published in final edited form as: Cell Rep. 2018 Feb 20;22(8):2080–2093. doi: 10.1016/j.celrep.2018.02.004

Figure 2. Microglia Hyper-proliferation and Induction of Lysosomal Genes in TSC1Cx3cr1CKO Mouse Brains.

Figure 2

(A) Confocal images acquired from CTX, CA1, CA3, and DG of WT and TSC1Cx3cr1CKO mouse brains. Scale bar, 10 μm.

(B and C) Quantification of Iba1-positive (B) and Iba1/CD68 double-positive (C) microglia in WT (n = 6; 3 males and 3 females) and TSC1Cx3cr1CKO (n = 7; 3 males and 4 females) mice.

(D) FACS analysis of CD68 expression in microglia prepared from WT and TSC1Cx3cr1CKO mouse brains.

(E) Quantification of the CD68-positive cell population in WT (n = 5; 2 males and 3 females) and TSC1Cx3cr1CKO (n = 5; 2 males and 3 females) mice. Data are presented as mean ± SEM (t test). ****p < 0.0001. See also Figure S2.

(F) Analysis of RNA-seq data using the iPathwayguide program revealed altered expression of lysosomal genes in TSC1Cx3cr1CKO microglia (log2 fold change compared to control). Data were from four sets of microglial preparations. p value (padj) < 0.05.