Table 9.
Genes identified as fitness factors for CAUTI by Tn-Seq that were also identified in the ascending UTI model by STM or transcriptomics
| Pathway | PMI number | Gene | Function | UTI transcriptome | UTI STM | CAUTI Tn-Seq |
|---|---|---|---|---|---|---|
| Amino acid transport and metabolism | PMI0145 | Hypothetical protein | Up | B | ||
| PMI0187 | dsdA | D-Serine ammonia-lyase | Up | B, K | ||
| PMI0347 | lysA | Diaminopimelate decarboxylase | Up | B, K | ||
| PMI0682 | artP | L-Arginine ABC transporter ATP-binding protein | Down | B | ||
| PMI0841 | lysP | Lysine:proton symporter, AAT family | Up | K | ||
| PMI1607 | sdaA | L-Serine ammonia-lyase | U | K | ||
| PMI1827 | cysK | Cysteine synthase | Up | K | ||
| PMI2093 | speB | Agmatinase | Down | K | ||
| PMI2094 | speA | Arginine decarboxylase | Down | B, K | ||
| PMI2259 | metN | D-Methionine ABC transporter, ATP-binding protein | Up | U, K | B, K | |
| PMI2736 | potB | Spermidine/putrescine ABC transporter, permease protein | Up | K | ||
| PMI2822 | pabA | Aminodeoxychorismate synthase, glutamine amidotransferase subunit | Up | B | ||
| PMI2882 | glnA | L-Glutamine synthetase | Down | B, K | ||
| PMI3052 | asnA | Aspartate-ammonia ligase | Up | B, K | ||
| Carbohydrate transport and metabolism | PMI0453 | nagA | N-Acetylglucosamine 6-phosphate deacetylase | Up | K | |
| PMI0454 | nagB | Glucosamine-6-phosphate deaminase | Up | B, K | ||
| PMI0873 | nagZ | β-N-Acetylhexosaminidase | Up | B | ||
| PMI1046 | Polysaccharide deacetylase | U, B, K | B, K | |||
| PMI1776 | PTS system IIB component, L-Asc family | Up | B | |||
| PMI1828 | hpr/ptsH | PTS system phosphocarrier protein | Up | B, K | ||
| PMI1829 | ptsI | Phosphoenolpyruvate--protein phosphotransferase | Up | B, K | ||
| PMI1830 | crr | PTS system D-glucose-specific IIA component, Glc family | Up | B, K | ||
| PMI1943 | MFS-family transporter | Down | B, K | |||
| PMI2134 | agaA | N-Acetylgalactosamine 6-phosphate deacetylase | Up | B | ||
| PMI2141 | agaR | Transcriptional regulator, DeoR family | Up | B, K | ||
| PMI2414 | deoB | Phosphopentomutase | Up | B, K | ||
| PMI3095 | N-Acetylglucosamine kinase | Up | K | |||
| PMI3716 | gpmB | Probable phosphoglycerate mutase | Up | K | ||
| Coenzyme transport and metabolism | PMI0105 | cyoE | Protoheme IX farnesyltransferase | Up | B, K | |
| PMI2022 | visC | 2-Octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase | Up | K | ||
| PMI2353 | ribB | 3,4-Dihydroxy-2-butanone 4-phosphate synthase | Down | K | ||
| PMI3547 | fre | NAD(P)H-flavin reductase | Up | B, K | ||
| Energy production and conversion | PMI0107 | cyoB | Cytochrome bo3 quinol oxidase subunit 1 apoprotein | Up | B, K | |
| PMI0108 | cyoA | Cytochrome bo3 quinol oxidase subunit 2 | Up | B, K | ||
| PMI0354 | nqrC | Na+-translocating NADH-quinone reductase subunit C | Up | B, K | ||
| PMI0355 | nqrD | Na+-translocating NADH-quinone reductase subunit D | Up | B, K | ||
| PMI0705 | pflB | Formate acetyltransferase | Up | K | ||
| PMI1200 | pntA | NAD(P) transhydrogenase subunit alpha (pyridine nucleotide transhydrogenase subunit alpha) | Up | B | ||
| PMI1753 | nuoK | NADH dehydrogenase subunit K | Up | B | ||
| PMI1754 | nuoJ | NADH dehydrogenase subunit J | Up | K | ||
| PMI1760 | nuoC/nuoD | NADH dehydrogenase subunit C (EC 1.6.5.3)/NADH dehydrogenase subunit D | Up | B, K | ||
| PMI1771 | ackA | Acetate kinase | Up | K | ||
| PMI1772 | pta | Phosphotransacetylase | Up | K | ||
| PMI2045 | aceF | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex | Up | B, K | ||
| PMI2046 | aceE | Pyruvate dehydrogenase E1 component | Up | B, K | B, K | |
| PMI2740 | qor | Quinone oxidoreductase | Up | K | ||
| PMI2930 | glpD/glyD | Homodimeric glycerol 3-phosphate dehydrogenase (quinone) | Down | B, K | ||
| PMI3108 | fdoI | Formate dehydrogenase (quinone-dependent) cytochrome b subunit | Up | K | ||
| PMI3300 | ilvM | Acetolactate synthase, small subunit | Up | K |