FIG. 1.

Cluster of islet-1-positive cells in C57BL/6J mouse heart. Immunostaining was performed at 1 day postnatal (a–c) and 7-month-old heart (d–f). The a and d panels show the entire heart section (at the depths of 535 and 470 μm, respectively) and the positive area is delimited (red square). The b and e panels show the clusters of islet-1-positive cells. The nuclear colocalization of islet-1 and DAPI signal is presented in c and f panels. Arrows indicate islet-1-positive cells. Scale bars: (a, d) 100 μm; (b, c, e, f) 50 μm. RA, right atria; LA, left atria; RV, right ventricle; IAS, interatrial septum; Ao, aorta; PA, pulmonary artery. Color images available online at www.liebertonline.com/scd