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. 2018 Apr 2;14(4):e1007291. doi: 10.1371/journal.pgen.1007291

Fig 3. SlaB downregulation results in ChsB depolarization.

Fig 3

(A) GFP-ChsB localization in hyphae derived from germlings that had been pre-cultured on medium containing nitrate as sole N source (left) and subsequently shifted to medium containing ammonium (right). (B) Promoter down-shift experiment with slaB1. The same nutritional regime used in (A) results in downregulation of SlaB levels (scheme), markedly affecting the polarization of GFP-ChsB in the PM. Class I (‘ruffled’) and class II (‘normal’) hyphae are depicted. For class I the inset shows a characteristic GFP-ChsB ‘clump’ associated with the PM (arrowed). (C) Quantitation of the perimeter of PM occupied by ChsB in wt (n = 19) and slaB1 (n = 15) cells pre-cultured on nitrate and shifted to ammonium. The two datasets were significantly different (P < 0.0001) in an unpaired t-test. All images represent MIPs of deconvolved z-stacks and are shown at the same magnification, with the exception of the inset, which is magnified 2.5 times.