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. 2018 Apr 2;14(4):e1007291. doi: 10.1371/journal.pgen.1007291

Fig 6. Dynamics of ChsB in the TGN.

Fig 6

(A) GFP-ChsB is a transient resident of TGN cisternae: kymograph traced along the longitudinal axis of a growing hypha showing multiple events of transient ChsB recruitment to TGN cisterna. The strong signal at the apex is the SPK. (B) Selected examples of events of ChsB recruitment to TGN cisternae and statistical analysis of the average residence time of ChsB on them (58 ± 4 S.D., n = 36); (C) region of a kymograph showing two examples of RabERAB11 being recruited at the end of the ‘ChsB cycle’. The green and red channels of a cell co-expressing mCh-ChsB and GFP-RabERAB11 were filmed simultaneously with a beam splitter at 1 fps time resolution (D) hypA1ts cells (hypA encodes A. nidulans Trs120 in TRAPPII) expressing GFP-ChsB were filmed at 28°C and at different times after shifting the culture to 37°C on the microscope stage. The time required for the culture medium to reach 37°C is ~15 min. Note the delocalization of ChsB at the apical dome and the SPK to internal structures.