Fig 9. RabC^RAB6- and GARP-dependent recycling of ChsB from an endosome located upstream of the RaB^RAB5 domain.

(A) Scheme of the effectors subordinated to RabB^RAB5 in early endosomes (EEs)(see text). (B) Normal localization of ChsB in a rabBΔ mutant. (C) Normal localization of ChsB in a vps33^ts strain at 28°C and following a shift to 42°C. (D) Left, delocalization of ChsB in rabCΔ; Middle, plot of average intensities of ChsB in wt and rabCΔ apical domes determined from 2 x 50 pixel arch-shaped linescans as in the scheme. P values estimated with an unpaired t-test; Right plot, linescans (mean values ± S.E.M. bars) of maximal intensities across the whole width of the same tips used for the plots. (E) Delocalization of ChsB from the apical dome in the vps52Δ mutant; middle and right plots and statistical analysis as in (D). Note that for (D) and (E), hyphal tip images were not deconvolved to better display the differences between the wt and the mutants.