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. 2018 Apr 2;14(4):e1007291. doi: 10.1371/journal.pgen.1007291

Fig 10. Characterization of A. nidulans GARP.

Fig 10

(A) Growth of the wt and indicated mutant strains after 60 h of incubation. (B) Silver staining of proteins retained after passing extracts of a strain expressing endogenously tagged Vps54-S-tag compared with an untagged strain control. Proteins were eluted from S-tag columns. The indicated bands were excised and their identity determined by MS/MS. (C) Comparison of S-tag affinity purifications as in (B) but using Vps54-S-tag and Vps51-S-tag baits and colloidal Coomassie staining. Note the shift in mobility of Vps51 due to the S-tag.