Fig. 3.

(A) The +1 PRF sites and surrounding regions. Multiple sequence alignment of regions encompassing the +1 PRF sites was performed using the 24 full-length amalgavirus ORF1+2 fusion protein sequences (Supplementary Fig. 1). The +1 PRF occurred at three positions which were labelled as #1, #2, and #3 and marked using red, green, and blue arrows, respectively. Number of viruses are shown in the parenthesis. The first aa residues of ORF2 are shown in colored boxes. (B) Sequence logo representations generated from all viruses (top), and those generated from viruses with the +1 PRF sites at position #1 (middle) and position #3 (bottom). Notably, the +1 PRF sites are embedded in a segment with diverse aa residues and surrounded by conserved regions.