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. 2001 Sep 18;98(20):11765–11770. doi: 10.1073/pnas.201394198

Figure 3.

Figure 3

(A) ERα protein expression in atria of sham-operated (sham), ovariectomized (ovx), and ovariectomized rats supplemented with 17-β-estradiol (ovxE). Twenty-five micrograms of protein homogenates of the left atrium (1) and right atrium (2) were subjected to SDS/PAGE, immunoblotted with an anti-ERα mAb, and visualized by the chemiluminescence technique. (B and C) RT-PCR analysis of ER transcripts and ANP in the left atrium (B) and right atrium (C) of sham-operated (sham), ovariectomized (ovx), and ovariectomized rats supplemented with 17-β-estradiol (ovxE). Total RNA (1 μg) was reverse-transcribed and cDNA was exponentially amplified in the presence of gene-specific primers. A PhosphorImager scan is presented after separation of the radioactive PCR product by electrophoresis in 1.5% agarose. Representative bands obtained in three RNA representative samples from sham, ovx, and ovxE rats are presented. The results were calculated as the ratio of the signal given by radioactive bands of ERα mRNA or ERβ mRNA to the corresponding 18S band. Values are means ± SEM adjusted to the values (in percentage) of sham-operated rats (100%). n = 6; *, P < 0.05 vs. sham; §, P < 0.05 vs. ovx.