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. 2018 Mar 21;14(3):e1007248. doi: 10.1371/journal.pgen.1007248

Fig 3. Degradation of GUS-CCT1 fusion proteins in FIN219-OE/GUS-CCT1 seedlings is mediated by 26S proteasome under blue light.

Fig 3

(A) Western blot analysis of GUS-CCT1 level in FIN219-OE/GUS-CCT1 seedlings transferred from the dark to blue light for different times (B15: 15 min blue light; B60: 60 min blue light) as shown above the figure. “+”: with MG132; “—”: without MG132. (B) Western blot analysis of GUS-CCT1 protein level in FIN219-OE/GUS-CCT1 seedlings transferred from the dark to blue light for different times shown above the figure. fin219-2/GUS-CCT1 seedlings were grown for 4 days under blue light. B1: 1-h blue light; B3: 3-h blue light; B24: 24-h blue light; B24M: 24-h blue light plus MG132; 4DB: 4 days of blue light.