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. 2018 Mar 21;14(3):e1007248. doi: 10.1371/journal.pgen.1007248

Fig 6. Changes in CRY1 levels affected MeJA-mediated physiological responses to various light conditions.

Fig 6

The cry1 mutant showed a hypersensitive response to MeJA-inhibited hypocotyl elongation under dark (A), low blue light (2 μmol•m-2•s-1) (B) and high blue light (15 μmol•m-2•s-1) as compared to the wild-type Col (C). Seedlings of wild-type Col, fin219-2, cry1, FIN219-OE, GUS-CCT1, FIN219-OE/GUS-CCT1, and fin219-2/GUS-CCT1 were grown on GM plates without or with 50 μM MeJA for 3 days, then hypocotyl elongation with MeJA was analyzed. Different lowercase letters represent significant differences by one-way ANOVA at P <0.05. (D) Ectopic expression of FIN219 reduced anthocyanin content in GUS-CCT1 seedlings under different light conditions, including the dark. Seedlings were grown in various light conditions for 3 days, then anthocyanin content was determined. Different lowercase letters represent significant differences by one-way ANOVA at P <0.05. (E) Ectopic expression of FIN219 reduced GUS-CCT1 seedling resistance to Pseudomonas syringae pv. tomato (Pst.) DC3000 infection. Leaves of 5-week-old Arabidopsis plants grown under short-day conditions were inoculated with Pst. DC3000. Phenotypic response was shown in top panel and quantification of the bacterial number in each sample was in lower panel. Different lowercase letters represent significant differences by one-way ANOVA at P <0.05.