Fig 7. GST6::luc promoter activity after treatment with thiol specific reagents.
GST6::luc seedlings grown in liquid MS medium (10 d old) were incubated with the thiol specific reagents (A) S-methyl methanethiosulfonate (MMTS), (B) iodoacetamide (IAM), (C) N-ethylmaleimide NEM and (D) diamide at different concentrations as indicated. Luminescence was measured 1–10 h after treatment. Symbols indicate mean values of three independent replicates ± SD. For comparison, black squares show the response to treatment with PGA1 (75 μM). Cartoons of reactions between proteins (black circles) and thiol-specific reagents are shown above the corresponding charts.