. 2018 Apr 2;13(4):e0195407. doi: 10.1371/journal.pone.0195407

Table 1. Primer sequences, annealing temperature (T_a) and amplicon size used for the sequencing and genetic diversity discovery at OXT locus in domestic camelids.

Region amplified	Primer	Sequence	T_a °C	Amplicon size
5’ Flanking Region	OXT 5end F	5’-AGCACCTCCCTGTTCAT-3’	65,3°C	957 bp
5’ Flanking Region	OXT prom R	5’-TGTCTAAGAGGGCCGC-3’	65,3°C	957 bp
Promoter–Exon 3	OXT prom F	5’-CGGCACTCGCTATCATC-3’	65,0°C	976 bp
Promoter–Exon 3	OXT Ex 3 R	5’-TCAGTGCTGGGAGAAGG	65,0°C	976 bp
3’ Flanking Region	OXT Ex 3 F	5’-CGCCGAGCCCGCCTG-3’	60,0°C	463 bp
3’ Flanking Region	OXT 3end R	5’-AGCCTTCTTCCCAATGTC-3’	60,0°C	463 bp

All the primers were designed using the contig_15904_16 as template (Genbank ID: LSZX01119753).

Table 1. Primer sequences, annealing temperature (Ta) and amplicon size used for the sequencing and genetic diversity discovery at OXT locus in domestic camelids.