Fig 1. α-Syn aggregates reduce selectively synaptic protein levels in cell-free systems.

(A) α-Syn aggregates were generated by incubation with dopamine at 37°C for 3 days followed by size-exclusion chromatography. (B) Transmission electron micrograph of α-syn aggregates showed the spherical shape and 10–20 nm in diameter. Scale bar, 100 nm. (C) Recombinant synaptic proteins (1 µM) were incubated with α-syn aggregates (100 nM) at 37°C for 2 h and subjected to western blot analysis or SyproOrange staining to detect protein levels. (D) Recombinant VAMP2 was incubated with α-syn aggregates at 37°C for 2 h. Western blot analysis using anti-VAMP2 (left) or anti-α-syn antibody (right), respectively showed a strong binding of VAMP2 to α-syn aggregates.