Fig 3. VAMP2 does not bind to α-syn monomer and Aβ aggregates.

Recombinant VAMP2 was incubated with the increasing concentration (0.1, 1, 10 µM) of α-syn monomer (A) or 100 nM Aβ aggregates (B) at 37°C for 2 h. Western blot analysis shows no binding of VAMP2 to α-syn monomer or Aβ aggregates. The weak band intensity of bound VAMP2 to α-syn aggregates was due to the saturated signals from free VAMP2.