Fig. 6.

Amino acid sequences of EH3 (EPHX3). On top is the sequence of human EH3. Below are three constructs of mouse EH3 prepared for testing in the current study, each with a N-terminal (His)₆ tag: i) truncated mouse EH3 with the rodent-specific 57 amino acid N-terminus removed; ii) truncated mouse EH3 containing a seven amino acid insert (VSVPPVK) at position 138; and iii) full-length mouse EH3. Arrowheads (with numbering of human EH3 residues) indicate the α/β-hydrolase catalytic triad (D173, D307, H337) and the two tyrosines (Y220, Y281) important for initial activation of the oxirane ring (14).